Thursday, November 8, 2012

Day 9 ...Awkward...

Today was a very interesting day. In the morning, I was in the final step of setting up electrophoresis to get the amounts of the certain genes, and I tripped with the sample in my hand. It fell on the ground and the samples left the wells. I had to start all over, which was really annoying and aggravating. I did eventually get it right and get some really good data. I should have finished ALL of the Trizol method cDNA by tomorrow. WOOT WOOT! Then I just have to do the cDNA from the Qaigen and I'll be done!

Also, I have taken my cell cultures and broken them down. Instead of being in a colony like they had been for the entire time, they've been detached from the vial and most were discarded. The only thing that remains is roughly 640000 cells that I will extract RNA from tomorrow and use Reverse Transcriptase to make cDNA!!!

About the title, today, Danny and I went to Grand Rounds. Usually, there is an interesting speaker and free pizza: DOUBLE WIN. But today, the speaker was terrible. He was SO BAD that the other attending doctors actually declared that his protocol was malpractice. Essentially, for twenty minutes, six doctors debated proper protocols for hyponatremia (lack of sodium in blood) in front of Danny, 50+ med students, and me... VERY AWKWARD!

2 comments:

  1. Pet peeve: "....in front of Danny, 50+ med students, and me! (not I)"

    Don't you think it is important that they called this guy's bluff? Better that the doctors speak up when they disagree with someone than that they sit quietly and let him confusing the interns.
    Mr. Boehm

    ReplyDelete
  2. No I agree that it's important to correct him for the med students, but it just was super awkward. Ask Danny.

    ReplyDelete